Acacb^tm1.2Lowl
Targeted Allele Detail

Summary

• Symbol: Acacb^tm1.2Lowl
• Name: acetyl-Coenzyme A carboxylase beta; targeted mutation 1.2, Bradford B Lowell
• MGI ID: MGI:4450934
• Synonyms: Acc2KO
• Gene: Acacb Location: Chr5:114284748-114388822 bp, + strand Genetic Position: Chr5, 55.97 cM
• Alliance: Acacb^tm1.2Lowl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159285
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: LoxP sites were positioned upstream of the preceding exon and downstream of the biotin binding domain exon using an Frt flanked kanamycin resistance cassette. The Frt-flanked selection cassette was removed by Flpe recombinase-mediated recombination. These two exons were deleted by Cre-mediated recombination, which introduces a frameshift and nonsense mutation after Asp865 in any translated protein. Immunoblotting showed no truncated protein expression in muscle lysates from homozygous mice. (J:159285)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Acacb Mutation: 109 strains or lines available

References

• Original: J:159285 Olson DP, et al., Gene knockout of Acc2 has little effect on body weight, fat mass, or food intake. Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7598-603
• All: 2 reference(s)