Clps^tm2.1Lowe
Targeted Allele Detail

Summary

• Symbol: Clps^tm2.1Lowe
• Name: colipase, pancreatic; targeted mutation 2.1, Mark E Lowe
• MGI ID: MGI:4450977
• Synonyms: Ent^-
• Gene: Clps Location: Chr17:28777184-28779740 bp, - strand Genetic Position: Chr17, 14.76 cM, cytoband B1
• Alliance: Clps^tm2.1Lowe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159587
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 15 base pairs in exon 1 encoding enterostatin, a pentapeptide (APGPR) produced from cleavage of the pro-colipase peptide, were removed. A floxed neo cassette was also inserted into intron 1 and subsequently removed by cre mediated recombination. Normal expression of a slightly smaller colipase protein was confirmed by western blot analysis on pancreas extracts. Enzymatic assay confirmed normal colipase activity. (J:159587)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clps Mutation: 11 strains or lines available

References

• Original: J:159587 Miller R, et al., Enterostatin deficiency increases serum cholesterol but does not influence growth and food intake in mice. Am J Physiol Endocrinol Metab. 2009 Oct;297(4):E856-65
• All: 1 reference(s)