Ciao2a^{tm2a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ciao2a^{tm2a(EUCOMM)Wtsi}
• Name: cytosolic iron-sulfur assembly component 2A; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4451949
• Gene: Ciao2a Location: Chr9:66033893-66046237 bp, + strand Genetic Position: Chr9, 35.73 cM, cytoband D
• Alliance: Ciao2a^{tm2a(EUCOMM)Wtsi} page
• IMPC: Ciao2a gene page

Mutation origin

• Mutant Cell Lines: EPD0538_2_A01, EPD0538_2_A02, EPD0538_2_A03, EPD0538_2_A04, EPD0538_2_A05, EPD0538_2_A08, EPD0538_2_A09, EPD0538_2_A10, EPD0538_2_A11, EPD0538_2_A12, EPD0538_2_B01, EPD0538_2_B02, EPD0538_2_B04, EPD0538_2_B05, EPD0538_2_B06, EPD0538_2_B07, EPD0538_2_B08, EPD0538_2_B09, EPD0538_2_B10, EPD0538_2_B11, EPD0538_2_B12, EPD0538_2_C01, EPD0538_2_C02, EPD0538_2_C03, EPD0538_2_C05, EPD0538_2_C06, EPD0538_2_C08, EPD0538_2_C10, EPD0538_2_C11, EPD0538_2_C12, EPD0538_2_D02, EPD0538_2_D03, EPD0538_2_D04, EPD0538_2_D05, EPD0538_2_D07, EPD0538_2_D10, EPD0538_2_D11, EPD0538_2_D12, EPD0538_2_E02, EPD0538_2_E04, EPD0538_2_E05, EPD0538_2_E06, EPD0538_2_E07, EPD0538_2_E09, EPD0538_2_E10, EPD0538_2_E12, EPD0538_2_F01, EPD0538_2_F02, EPD0538_2_F03, EPD0538_2_F04, EPD0538_2_F05, EPD0538_2_F07, EPD0538_2_F09, EPD0538_2_F10, EPD0538_2_F12, EPD0538_2_G01, EPD0538_2_G02, EPD0538_2_G03, EPD0538_2_G05, EPD0538_2_G06, EPD0538_2_G09, EPD0538_2_G10, EPD0538_2_H01, EPD0538_2_H02, EPD0538_2_H03, EPD0538_2_H04, EPD0538_2_H05, EPD0538_2_H06, EPD0538_2_H07, EPD0538_2_H08, EPD0538_2_H09, EPD0538_2_H10, EPD0538_2_H11, EPD0538_2_H12
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 66040225 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 66040999. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Ciao2a^tm2a(EUCOMM)Wtsi/Ciao2a^tm2a(EUCOMM)Wtsi	C57BL/6N-Ciao2a^tm2a(EUCOMM)Wtsi/Wtsi	EPD0538_2_A08

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms Sex:
Source:	IMPC - WTSI
hematopoietic system	√
decreased erythrocyte cell number	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Ciao2a Mutation: 15 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 5 reference(s)