Ascl1^{tm1.1(cre/ERT2)Jejo}
Targeted Allele Detail

Summary

• Symbol: Ascl1^{tm1.1(cre/ERT2)Jejo}
• Name: achaete-scute family bHLH transcription factor 1; targeted mutation 1.1, Jane Johnson
• MGI ID: MGI:4452601
• Synonyms: Ascl1-CreER, Ascl1^CreERT2
• Gene: Ascl1 Location: Chr10:87326681-87329522 bp, - strand Genetic Position: Chr10, 43.64 cM
• Alliance: Ascl1^{tm1.1(cre/ERT2)Jejo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171439
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Ascl1^{tm1.1(cre/ERT2)Jejo} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ascl1 (MGI:96919)

• Mutation details: A targeting vector was designed to replace the entire coding region of the achaete-scute complex homolog 1 (Ascl1 or Mash1) with a CreERT2 fusion protein and a frt-flanked neomycin (neo) selection cassette. The construct was electroporated into 129S/SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These mice were then bred to mice containing Flp-recombinase to remove the Frt-flanked sequence. These mice were then backcrossed at least 5 generations to and outbred ICR strain generate a colony of Ascl1-CreERT2 mice. (J:171439)

Generation of the Ascl1^{tm1.1(cre/ERT2)Jejo} allele

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ascl1 (mouse) Summary of all recombinase alleles driven by Ascl1. Comparative matrix view of recombinase activities Alliance mouse Ascl1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ascl1 Mutation: 28 strains or lines available

References

• Original: J:171439 Kim EJ, et al., Ascl1 (mash1) defines cells with long-term neurogenic potential in subgranular and subventricular zones in adult mouse brain. PLoS One. 2011;6(3):e18472
• All: 77 reference(s)