Smcr8^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Smcr8^{tm1(KOMP)Vlcg}
• Name: Smith-Magenis syndrome chromosome region, candidate 8 homolog (human); targeted mutation 1, Velocigene
• MGI ID: MGI:4452950
• Synonyms: Smcr8^betageo
• Gene: Smcr8 Location: Chr11:60668351-60679113 bp, + strand Genetic Position: Chr11, 37.83 cM
• Alliance: Smcr8^{tm1(KOMP)Vlcg} page
• IMPC: Smcr8 gene page

Mutation origin

• Mutant Cell Lines: 13849B-A5, 13849B-C1, 13849B-H2, 13849B-H8, 13849B-H9
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 2434 bp between positions 60668857-60671290 of Chromosome 11 (Build GRCm39). The gene locus is partially replaced by a cassette containing lacZ-polyA followed by a loxP-flanked hUbCpro-neo-polyA sequence. RT-PCR and Western blot analyses confirmed the absence of mRNA and protein expression in mouse embryonic fibroblasts (MEFs) derived from homozygous mutant embryos. (J:136110, J:236977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Smcr8 Mutation: 40 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 4 reference(s)