Arhgap35tm2Jset
Targeted Allele Detail
|
|
| Symbol: |
Arhgap35tm2Jset |
| Name: |
Rho GTPase activating protein 35; targeted mutation 2, Jeffrey Settleman |
| MGI ID: |
MGI:4453472 |
| Synonyms: |
p190RhoGAP null |
| Gene: |
Arhgap35 Location: Chr7:16228398-16349313 bp, - strand Genetic Position: Chr7, 9.15 cM
|
| Alliance: |
Arhgap35tm2Jset page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:160127
|
| Parent Cell Line: |
D3 (ES Cell)
|
| Strain of Origin: |
129S2/SvPas
|
|
| Allele Type: |
|
Targeted (Null/knockout) |
| Mutations: |
|
Insertion, Intragenic deletion
|
| |
|
Mutation details: The aim was to generate a novel mouse strain with a complete deletion of the p190RhoGAP-encoding gene. 1.5 kb of sequence, including the start codon and encoding the GTPase domain of exon 1, was replaced by a promoterless selection-reporter cassette encoding a Beta-Galactosidase-Neomycin resistance fusion protein in the sense orientation. The insertion site of the targeting vector is between a BamHI restriction site upstream of exon 1 and an XbaI site at the end of the replaced 1.5 kb portion. The Beta-Geo fusion protein expression is totally dependent on the endogenous promoter. The disrupted gene does not have any detectable protein products. The primary sequence of the mutant allele was depositied in the GenBank database (www.ncbi.nlm.nih.gov/genbank) under
accession number HM365221.
(J:162994)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Arhgap35 Mutation: |
70 strains or lines available
|
|
| Original: |
J:160127 Nemeth T, Targeted mutation of the glucocorticoid receptor DNA binding factor 1. MGI Direct Data Submission. 2010; |
| All: |
2 reference(s) |
|
Analysis Tools
