Tg(Slc6a5-icre)121Veul
Transgene Detail

Summary

• Symbol: Tg(Slc6a5-icre)121Veul
• Name: transgene insertion 121, Volker Eulenburg
• MGI ID: MGI:4453975
• Synonyms: GlyT2-Cre
• Transgene: Tg(Slc6a5-icre)121Veul Location: unknown
• Alliance: Tg(Slc6a5-icre)121Veul page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Slc6a5-icre)121Veul expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Slc6a5

• Mutation details: A BAC containing all coding exons of Slc6a5 and the 5' UTR was modified using a 2-step homologous recombination method resulting in the coding sequence for a codon-optimized cre (icre) including the nuclear localization signal being inserted in frame into exon 2. The modifed BAC was linearized and injected into B6D2F1 single cell zygotes. Seven founder which transmitted the transgene were obtained and five lines were analyzed (lines 118 (5 transgene copies), 121 (1 copy), 122.2 (1 copy), 122.4 (1 copy), and 128 (2 copies)). Line 121 was analyzed in greater detail. (J:160216)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc6a5 (species not specified) Summary of all recombinase alleles driven by Slc6a5. Comparative matrix view of recombinase activities

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:160216 Ishihara N, et al., Generation of a mouse line expressing Cre recombinase in glycinergic interneurons. Genesis. 2010 Jul;48(7):437-45
• All: 3 reference(s)