Plpp5^tm1(KOMP)Mbp
Targeted Allele Detail

Summary

• Symbol: Plpp5^tm1(KOMP)Mbp
• Name: phospholipid phosphatase 5; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:4453994
• Synonyms: Plpp5^-
• Gene: Plpp5 Location: Chr8:26210064-26214914 bp, + strand Genetic Position: Chr8, 14.17 cM, cytoband A3
• Alliance: Plpp5^tm1(KOMP)Mbp page
• IMPC: Plpp5 gene page

Mutation origin

• Mutant Cell Lines: DEPD00512_4_A01, DEPD00512_4_A02, DEPD00512_4_A03, DEPD00512_4_A04, DEPD00512_4_B02, DEPD00512_4_B03, DEPD00512_4_B04, DEPD00512_4_C01, DEPD00512_4_C02, DEPD00512_4_C03, DEPD00512_4_C04, DEPD00512_4_D01, DEPD00512_4_D02, DEPD00512_4_E01, DEPD00512_4_E02, DEPD00512_4_F02, DEPD00512_4_F03, DEPD00512_4_F04, DEPD00512_4_G01, DEPD00512_4_G04, DEPD00512_4_H02, DEPD00512_4_H04
• Germline Transmission: Earliest citation of germline transmission: J:265892
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 2350 starting at position 26210291 and ending at position 26212641 of Chromosome 8 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064, J:265892)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Plpp5 Mutation: 12 strains or lines available

Notes

Cell line DEPD00512_4_E01 was successfully used to make chimeric mice. Germline transmission was accomplished. J:265892

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 2 reference(s)