Megf10^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Megf10^{tm1(KOMP)Vlcg}
• Name: multiple EGF-like-domains 10; targeted mutation 1, Velocigene
• MGI ID: MGI:4454190
• Gene: Megf10 Location: Chr18:57266162-57430539 bp, + strand Genetic Position: Chr18, 31.5 cM
• Alliance: Megf10^{tm1(KOMP)Vlcg} page
• IMPC: Megf10 gene page

Mutation origin

• Mutant Cell Lines: 14914B-B12, 14914C-F4
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 31918bp between positions 57313561-57345478 of Chromosome 18 (Build GRCm39). This deletes exons 1-24. Quantitative PCR confirmed absence of RNA in the cerebellum of homozygotes. (J:136110, J:231811)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Megf10 Mutation: 69 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 5 reference(s)