Tg(Aire-cre)410-5Mmat
Transgene Detail

Summary

• Symbol: Tg(Aire-cre)410-5Mmat
• Name: transgene insertion 410-5, Mitsuru Matsumoto
• MGI ID: MGI:4454269
• Synonyms: Aire/Cre BAC Tg line 410-5
• Transgene: Tg(Aire-cre)410-5Mmat Location: unknown
• Alliance: Tg(Aire-cre)410-5Mmat page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Aire-cre)410-5Mmat expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Aire (MGI:1338803)

• Mutation details: The BAC RP23-461E7 containing the Aire locus was modified to replace the Aire start codon with the open reading frame of cre recombinase followed by the rabbit beta-globin polyA signal, using the Red/ET Counter Selection BAC Modification kit. The linearized BAC construct was injected into pronuclei of fertilized C57BL/6 oocytes. Multiple founders were generated. When crossed to a reporter mouse strain, lines410-4, 413-3, 4155-2, and 461-1 displayed GFP expression in the entire thymus region, as well as in all tissues examined (as result of embryonic expression in all three germ layers.) GFP expression was confined to particular thymic components or cells in two other lines (410-3 and 410-5). Line 410-5 was characterized in more detail, and two distinct patterns of expression were observed. (J:160217)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Aire (mouse) Summary of all recombinase alleles driven by Aire. Comparative matrix view of recombinase activities Alliance mouse Aire gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:160217 Nishikawa Y, et al., Biphasic Aire expression in early embryos and in medullary thymic epithelial cells before end-stage terminal differentiation. J Exp Med. 2010 May 10;207(5):963-71
• All: 1 reference(s)