Patz1^tm1.1Welm
Targeted Allele Detail

Summary

• Symbol: Patz1^tm1.1Welm
• Name: POZ (BTB) and AT hook containing zinc finger 1; targeted mutation 1.1, Wilfried Ellmeier
• MGI ID: MGI:4454313
• Gene: Patz1 Location: Chr11:3239131-3259083 bp, + strand Genetic Position: Chr11, 2.3 cM
• Alliance: Patz1^tm1.1Welm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158947
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette was inserted within intron 1 and a single loxP site 5' to exon 1 by homologous recombination. Exon 1 and the neo cassette were removed by cre mediated recombination. (J:158947)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Patz1 Mutation: 55 strains or lines available

Notes

From: Wilfried Ellmeier [wilfried.ellmeier@meduniwien.ac.at] Sent: Monday, May 24, 2010 2:24 PM To: Jason Bubier Cc: Wilfried Ellmeier Subject: Re: Information Request Follow Up Flag: Follow Up Flag Status: Flagged Dear Dr. Bubier, Sorry for the delayed reply. We used the widely used E14 ES cell line for the deletion. The floxed MAZR allele was deleted in ES cells with CMV-Cre (using transient transfection), therefore we generated a germ-line knockout. With best wishes, Wilfried Ellmeier

References

• Original: J:158947 Sakaguchi S, et al., The zinc-finger protein MAZR is part of the transcription factor network that controls the CD4 versus CD8 lineage fate of double-positive thymocytes. Nat Immunol. 2010 May;11(5):442-8
• All: 3 reference(s)