Il4ra^tm3.1Tch
Targeted Allele Detail

Summary

• Symbol: Il4ra^tm3.1Tch
• Name: interleukin 4 receptor, alpha; targeted mutation 3.1, Talal A Chatila
• MGI ID: MGI:4455123
• Synonyms: Il4ra^F709, IL-4Ra Y709F
• Gene: Il4ra Location: Chr7:125151443-125178646 bp, + strand Genetic Position: Chr7, 68.94 cM
• Alliance: Il4ra^tm3.1Tch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:160806
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Two base pair substitutions (A->T and C->T at basepair 2362 and 2363, respectively) were introduced into the murine Il4ra cDNA using site-directed mutagenesis. These mutations result in a single amino acid substitution of tyrosine to phenylalanine at codon 709 (Y709F) within the canonical immunoreceptor tyrosine-based inhibitory motif (ITIM) sequence near the carboxyl-terminus of the protein. The targeting vector included these two mutations and a loxP-flanked neo cassette inserted into intron 11. Cre mdiated recombination removed the neo cassette. (J:160806)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il4ra Mutation: 46 strains or lines available

References

• Original: J:160806 Tachdjian R, et al., In vivo regulation of the allergic response by the IL-4 receptor alpha chain immunoreceptor tyrosine-based inhibitory motif. J Allergy Clin Immunol. 2010 May;125(5):1128-1136.e8
• All: 9 reference(s)