Wdfy3^{tm1a(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Wdfy3^{tm1a(KOMP)Mbp}
• Name: WD repeat and FYVE domain containing 3; targeted mutation 1a, Mouse Biology Program, UC Davis
• MGI ID: MGI:4455365
• Synonyms: Wdfy3^lacZ
• Gene: Wdfy3 Location: Chr5:101980822-102217787 bp, - strand Genetic Position: Chr5, 48.95 cM
• Alliance: Wdfy3^{tm1a(KOMP)Mbp} page
• IMPC: Wdfy3 gene page

Wdfy3^{tm1a(KOMP)Mbp}/Wdfy3^{tm1a(KOMP)Mbp} mice exhibit severe neurodevelopmental defects

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: DEPD00523_5_A09, DEPD00523_5_A10, DEPD00523_5_A11, DEPD00523_5_A12, DEPD00523_5_B09, DEPD00523_5_B10, DEPD00523_5_B11, DEPD00523_5_C10, DEPD00523_5_C11, DEPD00523_5_C12, DEPD00523_5_D09, DEPD00523_5_D10, DEPD00523_5_D11, DEPD00523_5_D12, DEPD00523_5_E09, DEPD00523_5_E10, DEPD00523_5_E11, DEPD00523_5_F10, DEPD00523_5_F11, DEPD00523_5_F12, DEPD00523_5_G09, DEPD00523_5_G10, DEPD00523_5_G11, DEPD00523_5_H10, DEPD00523_5_H12
• Germline Transmission: Earliest citation of germline transmission: J:225200
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Conditional ready, Hypomorph, Reporter) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 102100564 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 102101479. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Generation of the Wdfy3^tm1a(KOMP)Mbp and Wdfy3^tm1c(KOMP)Mbp alleles

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Wdfy3 Mutation: 210 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 6 reference(s)