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Scapertm1a(EUCOMM)Hmgu
Targeted Allele Detail
Summary
Symbol: Scapertm1a(EUCOMM)Hmgu
Name: S phase cyclin A-associated protein in the ER; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4455688
Gene: Scaper  Location: Chr9:55457163-55845403 bp, - strand  Genetic Position: Chr9, 30.13 cM
Alliance: Scapertm1a(EUCOMM)Hmgu page
IMPC: Scaper gene page
Mutation
origin
Mutant Cell Lines:  HEPD0629_8_A04, HEPD0629_8_C04, HEPD0629_8_G04, HEPD0629_8_H01, HEPD0629_8_H02, HEPD0654_7_A07, HEPD0654_7_A08, HEPD0654_7_B05, HEPD0654_7_B07, HEPD0654_7_C05, HEPD0654_7_C06, HEPD0654_7_C07, HEPD0654_7_C08, HEPD0654_7_D05, HEPD0654_7_D06, HEPD0654_7_D07, HEPD0654_7_D08, HEPD0654_7_E05, HEPD0654_7_E06, HEPD0654_7_E07, HEPD0654_7_E08, HEPD0654_7_F05, HEPD0654_7_F07, HEPD0654_7_H07, HEPD0654_7_H08
Germline Transmission:  Earliest citation of germline transmission: J:294607
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 55821961 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 55822706. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:294607)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 7 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Scaper Mutation:  82 strains or lines available
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory