Actb^{tm3.1(Sirt1)Npa}
Targeted Allele Detail

Summary

• Symbol: Actb^{tm3.1(Sirt1)Npa}
• Name: actin, beta; targeted mutation 3.1, Novartis Pharma AG
• MGI ID: MGI:4456082
• Synonyms: SIRT1-KI⁺
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^{tm3.1(Sirt1)Npa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:160212
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Actb^{tm3.1(Sirt1)Npa} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sirt1 (MGI:2135607)

• Mutation details: The 3' UTR was replaced with a cassette containing an IRES sequence, floxed neo cassette, mouse Sirt1 cDNA, and a bovine growth hormone polyadenylation sequence. Cre mediated recombination removed the neo cassette. Western blot analysis confirmed expression of the cDNA in white adipose tissue, brown adipose tissue, brain, calvaria cells of the skull, and mouse embryonic fibroblasts but not in the liver or muscle. Normal expression of Actb was confirmed by western blot analysis. (J:160212)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 53 strains or lines available

References

• Original: J:160212 Bordone L, et al., SIRT1 transgenic mice show phenotypes resembling calorie restriction. Aging Cell. 2007 Dec;6(6):759-67
• All: 11 reference(s)