Tg(ACTB-SLC2A1)224Cwh
Transgene Detail

Summary

• Symbol: Tg(ACTB-SLC2A1)224Cwh
• Name: transgene insertion 224, Charles W Heilig
• MGI ID: MGI:4456414
• Synonyms: GT1S (Line S224)
• Transgene: Tg(ACTB-SLC2A1)224Cwh Location: unknown
• Alliance: Tg(ACTB-SLC2A1)224Cwh page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(ACTB-SLC2A1)224Cwh expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SLC2A1 (6513)

  Slc2a1

  (MGI:95755)

• Mutation details: This transgene contains a full length human cDNA encoding solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1; also called glucose transporter 1, GLUT1) between a modified human beta-actin promoter and the SV40 polyadenylation signal. Western blot analysis demonstrates 3.5-fold nomal levels of glomerular GLUT1 protein in 26 week old mice hemizygous for the transgene. Immunolabeling detects GLUT1 overexpression in glomerular mesagial cells and small vessels, but not in the renal tubules, of the kidney. (J:160943)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:160943 Wang Y, et al., Transgenic overexpression of GLUT1 in mouse glomeruli produces renal disease resembling diabetic glomerulosclerosis. Am J Physiol Renal Physiol. 2010 Jul;299(1):F99-F111
• All: 1 reference(s)