Spag4^tm1(KOMP)Mbp
Targeted Allele Detail

Summary

• Symbol: Spag4^tm1(KOMP)Mbp
• Name: sperm associated antigen 4; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:4456603
• Synonyms: Spag4^-, Sun4^-
• Gene: Spag4 Location: Chr2:155907108-155911421 bp, + strand Genetic Position: Chr2, 77.34 cM
• Alliance: Spag4^tm1(KOMP)Mbp page
• IMPC: Spag4 gene page

Mutation origin

• Mutant Cell Lines: DEPD00501_2_A01, DEPD00501_2_A02, DEPD00501_2_A03, DEPD00501_2_A04, DEPD00501_2_B01, DEPD00501_2_B03, DEPD00501_2_B04, DEPD00501_2_C01, DEPD00501_2_C02, DEPD00501_2_C03, DEPD00501_2_C04, DEPD00501_2_D02, DEPD00501_2_E01, DEPD00501_2_E02, DEPD00501_2_E03, DEPD00501_2_F01, DEPD00501_2_F02, DEPD00501_2_F04, DEPD00501_2_G01, DEPD00501_2_G03, DEPD00501_2_H04
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 2456 starting at position 155908044 and ending at position 155910500 of Chromosome 2 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064, J:234041)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Spag4 Mutation: 17 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 5 reference(s)