Gfer^tm1(KOMP)Mbp
Targeted Allele Detail

Summary

• Symbol: Gfer^tm1(KOMP)Mbp
• Name: growth factor, augmenter of liver regeneration; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:4456605
• Synonyms: ALR^-, ALR-KO, Gfer^-
• Gene: Gfer Location: Chr17:24912164-24915065 bp, - strand Genetic Position: Chr17, 12.48 cM
• Alliance: Gfer^tm1(KOMP)Mbp page
• IMPC: Gfer gene page

Mutation origin

• Mutant Cell Lines: DEPD00501_2_A05, DEPD00501_2_A06, DEPD00501_2_A07, DEPD00501_2_A08, DEPD00501_2_B05, DEPD00501_2_B06, DEPD00501_2_B07, DEPD00501_2_C06, DEPD00501_2_C08, DEPD00501_2_D05, DEPD00501_2_D06, DEPD00501_2_D07, DEPD00501_2_D08, DEPD00501_2_E05, DEPD00501_2_E06, DEPD00501_2_E08, DEPD00501_2_F05, DEPD00501_2_F07, DEPD00501_2_F08, DEPD00501_2_G05, DEPD00501_2_G06, DEPD00501_2_G07, DEPD00501_2_G08, DEPD00501_2_H05, DEPD00501_2_H06, DEPD00501_2_H08
• Germline Transmission: Earliest citation of germline transmission: J:224792
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 1250 starting at position 24913203 and ending at position 24914453 of Chromosome 17 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Gfer Mutation: 22 strains or lines available

Notes

Cell line DEPD00501_2_D08 was successfully used to make chimeric mice. Germline transmission was accomplished. J:224792

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 3 reference(s)