Dgcr8^tm1.1Blel
Targeted Allele Detail

Summary

• Symbol: Dgcr8^tm1.1Blel
• Name: DGCR8, microprocessor complex subunit; targeted mutation 1.1, Robert Blelloch
• MGI ID: MGI:4458072
• Synonyms: Dgcr^2lox
• Gene: Dgcr8 Location: Chr16:18071812-18107110 bp, - strand Genetic Position: Chr16, 11.31 cM, cytoband B1
• Alliance: Dgcr8^tm1.1Blel page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:161342
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted in intron 2 and a floxed CMV-Hygro selection cassette was inserted in intron 3. ES cells were sequentially targeted so that ES cells carried two mutated alleles. Cre recombinase treatment of these ES cells resulted in two mutant alleles, this one with a floxed exon 3 but lacking the selection cassette and another allele which lacks both exon 3 and the selection cassette. (J:141670)

Expression

In Mice Carrying this Mutation:	8 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dgcr8 Mutation: 67 strains or lines available

References

• Original: J:161342 Rao PK, et al., Loss of cardiac microRNA-mediated regulation leads to dilated cardiomyopathy and heart failure. Circ Res. 2009 Sep 11;105(6):585-94
• All: 48 reference(s)