Ehd1^tm1.2Haba
Targeted Allele Detail

Summary

• Symbol: Ehd1^tm1.2Haba
• Name: EH-domain containing 1; targeted mutation 1.2, Hamid Band
• MGI ID: MGI:4458098
• Synonyms: Ehd1^-
• Gene: Ehd1 Location: Chr19:6326926-6350126 bp, + strand Genetic Position: Chr19, 4.4 cM
• Alliance: Ehd1^tm1.2Haba page

Ehd1^tm1.2Haba/Ehd1^tm1.2Haba mice are small and males exhibit small testis

Show the 5 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:160452
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: Two loxP sites were introduced to flank exon 1 by recombineering. An engineered FRT-Neo-FRT selection cassette was immediately preceding the second loxP site. The first exon was deleted by Cre-mediated recombination. RT-PCR and immunostaining confirmed the absence of mRNA and protein expression in the testis of homozygous mice. Western blots performed on lysates of mouse lung, kidney, heart, spleen, and testis confirmed the loss of the protein expression. (J:160452)

Generation of the Ehd1^tm1.2Haba and Ehd1^tm1.1Haba alleles

Expression

In Mice Carrying this Mutation:	47 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ehd1 Mutation: 40 strains or lines available

References

• Original: J:160452 Rainey MA, et al., The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice. BMC Dev Biol. 2010;10:37
• All: 7 reference(s)