Braf^tm1Rima
Targeted Allele Detail

Summary

• Symbol: Braf^tm1Rima
• Name: Braf transforming gene; targeted mutation 1, Richard Marais
• MGI ID: MGI:4458346
• Synonyms: Braf^LSL-D594A, LSL-Braf^D594A
• Gene: Braf Location: Chr6:39580171-39702397 bp, - strand Genetic Position: Chr6, 18.43 cM
• Alliance: Braf^tm1Rima page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:161180
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 15 was replaced with one containing nucleotide substitutions that result in the amino acid substitution of alanine for aspartic acid at position 594 (D594). The modified exon 15 is preceded by a loxP site, a cDNA encoding exons 15 through 18, a termination sequence, and a floxed neo cassette. (J:161180)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Braf Mutation: 60 strains or lines available

References

• Original: J:161180 Heidorn SJ, et al., Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF. Cell. 2010 Jan 22;140(2):209-21
• All: 4 reference(s)