Il13^{tm1(YFP/cre)Lky}
Targeted Allele Detail

Summary

• Symbol: Il13^{tm1(YFP/cre)Lky}
• Name: interleukin 13; targeted mutation 1, Richard M Locksley
• MGI ID: MGI:4459318
• Synonyms: YetCre-13
• Gene: Il13 Location: Chr11:53522151-53525529 bp, - strand Genetic Position: Chr11, 31.98 cM
• Alliance: Il13^{tm1(YFP/cre)Lky} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:161387
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (No functional change, Recombinase, Reporter)
• Mutation: Insertion
• Il13^{tm1(YFP/cre)Lky} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Il13 (MGI:96541)

• Mutation details: A targeting construct containing a bicistronic reporter/recombinase cassette (loxP-Neo-loxP IRES-YFP-cre cassette and DTA negative-selection cassette with appropriate homology arms) was electroporated into ES cells containing a Prm-cre transgene. Homologous recombination resulted in insertion of the IRES-YFP-cre sequence between the translational stop codon and the 3'UTR of the Il13 gene, preserving the normal function of the gene yet allowing expression of the YFP/cre fusion protein. The floxed Neo cassette was deleted in the male germline (by Prm-cre) when chimeras were bred to wild-type females. (J:161387)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Il13 (mouse) Summary of all recombinase alleles driven by Il13. Alliance mouse Il13 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il13 Mutation: 30 strains or lines available

References

• Original: J:161387 Price AE, et al., Systemically dispersed innate IL-13-expressing cells in type 2 immunity. Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11489-94
• All: 21 reference(s)