Ap5z1^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ap5z1^{tm1(KOMP)Wtsi}
• Name: adaptor-related protein complex 5, zeta 1 subunit; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4460281
• Synonyms: AP5 zeta KO
• Gene: Ap5z1 Location: Chr5:142449699-142464465 bp, + strand Genetic Position: Chr5, 81.53 cM
• Alliance: Ap5z1^{tm1(KOMP)Wtsi} page
• IMPC: Ap5z1 gene page

Mutation origin

• Mutant Cell Lines: EPD0587_5_A05, EPD0587_5_A06, EPD0587_5_A08, EPD0587_5_B06, EPD0587_5_B07, EPD0587_5_B08, EPD0587_5_C08, EPD0587_5_D08, EPD0587_5_E06, EPD0587_5_E07, EPD0587_5_E08, EPD0587_5_F06, EPD0587_5_G06, EPD0587_5_H05, EPD0587_5_H06, EPD0587_5_H07
• Germline Transmission: Earliest citation of germline transmission: J:283611
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 3956 starting at position 142451972 and ending at position 142455928 of Chromosome 5 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Ap5z1 Mutation: 42 strains or lines available

Notes

Cell line EPD0587_5_A05 was successfully used to make chimeric mice. Germline transmission was accomplished. J:283611

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 6 reference(s)