Krt9^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Krt9^{tm1(KOMP)Vlcg}
• Name: keratin 9; targeted mutation 1, Velocigene
• MGI ID: MGI:4460538
• Gene: Krt9 Location: Chr11:100077607-100084072 bp, - strand Genetic Position: Chr11, 63.43 cM
• Alliance: Krt9^{tm1(KOMP)Vlcg} page
• IMPC: Krt9 gene page

Mutation origin

• Mutant Cell Lines: 10313A-A9, 10313A-A11, 10313A-B1, 10313A-C1, 10313A-C10, 10313A-E7
• Germline Transmission: Earliest citation of germline transmission: J:206051
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 6142bp between positions 100077885-100084026 of Chromosome 11 (Build GRCm39). The cassette replaced the coding sequence. Immunoblotting confirmed the absence of protein expression in the plantar epidermis. (J:136110, J:206051)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Krt9 Mutation: 50 strains or lines available

Notes

Cell line 10313A-C10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:206051

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 3 reference(s)