Elfn2^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Elfn2^{tm1(KOMP)Vlcg}
• Name: leucine rich repeat and fibronectin type III, extracellular 2; targeted mutation 1, Velocigene
• MGI ID: MGI:4460634
• Gene: Elfn2 Location: Chr15:78551531-78602971 bp, - strand Genetic Position: Chr15, 37.64 cM
• Alliance: Elfn2^{tm1(KOMP)Vlcg} page
• IMPC: Elfn2 gene page

Mutation origin

• Mutant Cell Lines: 14598A-A3, 14598A-B10, 14598A-F9, 14598A-G4, 14598A-H3
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 2488bp between positions 78556055-78558542 of Chromosome 15 (Build GRCm39). Exon 2 was replaced with a lacZ-neo cassette while preserving the endogenous translational initiation codon. Western blot analysis of whole brain lysates confirmed the complete absence of encoded protein in homozygous mutant mice. (J:136110, J:286127)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Elfn2 Mutation: 29 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 4 reference(s)