Mir140^tm1.1Asah
Targeted Allele Detail

Summary

• Symbol: Mir140^tm1.1Asah
• Name: microRNA 140; targeted mutation 1.1, Hiroshi Asahara
• MGI ID: MGI:4461000
• Synonyms: pre-miR-140-null
• Gene: Mir140 Location: Chr8:108277876-108277945 bp, + strand Genetic Position: Chr8, 54.33 cM
• Alliance: Mir140^tm1.1Asah page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:160358
• Parent Cell Line: TT2F (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire pre-microRNA sequence was replaced with a floxed neo cassette. Cre mediated recombination removed the neo cassette. QPCR confirmed the absence of transcript expression in E11.5 embryo extracts. (J:160358)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir140 Mutation: 3 strains or lines available

References

• Original: J:160358 Miyaki S, et al., MicroRNA-140 plays dual roles in both cartilage development and homeostasis. Genes Dev. 2010 Jun 1;24(11):1173-85
• All: 4 reference(s)