Tg(RP23-326M22)760She
Transgene Detail

Summary

• Symbol: Tg(RP23-326M22)760She
• Name: transgene insertion 760, Sarah Elsea
• MGI ID: MGI:4461458
• Synonyms: Rai1 BAC transgene, Rai1 transgene, TgRai1, Tg(RP23-326M22)1She
• Transgene: Tg(RP23-326M22)760She Location: unknown
• Alliance: Tg(RP23-326M22)760She page

Transgene origin

• Strain of Origin: (C57BL/6J x SJL)F2 or C57BL/6J x (C57BL/6J x SJL)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(RP23-326M22)760She expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Rai1 (MGI:103291)
  mouse	Srebf1 (MGI:107606)

• Mutation details: This transgene consists of the C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-326M22 (GenBank id AC096624), which contains the entire coding sequence of the retinoic acid induced 1 (Rai1) gene. The BAC also includes the sterol regulator element binding transcription factor 1 gene (Srebf1; formerly Srebp1) and the 3' end of Tom1l2. Founder line 760 has integrated ~4 copies of the transgene at a single site. Overexpression of Rai1 in the brain and liver was verified by quantitative RT-PCR and western blot analysis. (J:161765)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

A second transgenic line generated using the same BAC was called Tg(RP23-326M22)775She (or Tg(RP23-326M22)2She). "All reported data were generated from the 760 line; however, the 775 line of mice showed similar results." (J:161765)

References

• Original: J:161765 Girirajan S, et al., How much is too much? Phenotypic consequences of Rai1 overexpression in mice. Eur J Hum Genet. 2008 Aug;16(8):941-54
• All: 2 reference(s)