Cbfb^tm3Ppl
Targeted Allele Detail

Summary

• Symbol: Cbfb^tm3Ppl
• Name: core binding factor beta; targeted mutation 3, Pu P Liu
• MGI ID: MGI:4461804
• Synonyms: Cbfb^MYH11 (d179-221)
• Gene: Cbfb Location: Chr8:105897306-105944621 bp, + strand Genetic Position: Chr8, 53.04 cM
• Alliance: Cbfb^tm3Ppl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:160522
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 5 was replaced by a construct composed of the first 25 bp of mouse exon 5 fused to the 3' end of a human CBFB/MYH11 cDNA. The human cDNA fragment used contained the last 66 bp of human exon 5 and exons 33-42 of MYH11. The sequence in exon 33 encoding amino acids 179-221 are deleted. A bovine growth hormone polyadenylation site and a floxed PGK-neomycin cassette were inserted immediately downstream. (J:160522)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cbfb Mutation: 36 strains or lines available

References

• Original: J:160522 Kamikubo Y, et al., Accelerated leukemogenesis by truncated CBF beta-SMMHC defective in high-affinity binding with RUNX1. Cancer Cell. 2010 May 18;17(5):455-68
• All: 1 reference(s)