Hprt1^tm1Dwm
Targeted Allele Detail

Summary

• Symbol: Hprt1^tm1Dwm
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, David W Melton
• MGI ID: MGI:4462242
• Synonyms: Hprt^b-m4
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^tm1Dwm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:161048
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A vector was inserted in exon 3 that replaces the promoter, exon 1, and exon 2 that are missing in the endogenous gene in ES cell line E14TG2a. Correct targeting was expected to result in a duplicate copy of exon 3 located 5' of the promoter. In the specific clone used this duplicated exon was deleted resulting in restoration of the functional gene structure. Northern blot analysis confirmed that the corrected transcript was produced. (J:161048)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:161048 Thompson S, et al., Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells. Cell. 1989 Jan 27;56(2):313-21
• All: 1 reference(s)