Sod2^{tm1(tetO-Sod2)Evbo}
Targeted Allele Detail

Summary

• Symbol: Sod2^{tm1(tetO-Sod2)Evbo}
• Name: superoxide dismutase 2, mitochondrial; targeted mutation 1, Eva Bober
• MGI ID: MGI:4818481
• Gene: Sod2 Location: Chr17:13226726-13237006 bp, + strand Genetic Position: Chr17, 8.75 cM
• Alliance: Sod2^{tm1(tetO-Sod2)Evbo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:162190
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Null/knockout, Reporter)
• Inducer: doxycycline/tetracycline
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 5'UTR, exon 1, and exon 2 wwere replaced with a construct containing (5' to 3') an frt flanked neo cassette, tetracycline responsive (tetO/TRE) promoter, a myc-tagged Sod2 cDNA, and an IRES-EGFP. The absence of protein expression was confirmed by western blot analysis on embryonic fibroblast. (J:162190)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sod2 Mutation: 26 strains or lines available

References

• Original: J:162190 Loch T, et al., Different extent of cardiac malfunction and resistance to oxidative stress in heterozygous and homozygous manganese-dependent superoxide dismutase-mutant mice. Cardiovasc Res. 2009 Jun 1;82(3):448-57
• All: 2 reference(s)