Jak2^tm1.1Ble
Targeted Allele Detail

Summary

• Symbol: Jak2^tm1.1Ble
• Name: Janus kinase 2; targeted mutation 1.1, Benjamin L Ebert
• MGI ID: MGI:4818800
• Synonyms: Jak2^Fl
• Gene: Jak2 Location: Chr19:29229228-29290480 bp, + strand Genetic Position: Chr19, 23.73 cM
• Alliance: Jak2^tm1.1Ble page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:160883
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 14 and a cassette, consisting of a loxP511 site, a linker, an inverted loxP site, an inverted exon 14 containing a G1849T mutation, an inverted loxP511 site, and an FRT flanked neo selection cassette, was inserted downstream of exon 14 via homologous recombination. The neo cassette was removed by flp mediated recombination. The targeting cassette appears to interfere with endogenous gene expression making this a null allele. (J:160883)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Jak2 Mutation: 59 strains or lines available

References

• Original: J:160883 Mullally A, et al., Physiological Jak2V617F expression causes a lethal myeloproliferative neoplasm with differential effects on hematopoietic stem and progenitor cells. Cancer Cell. 2010 Jun 15;17(6):584-96
• All: 8 reference(s)