Itga5^tm2Hyn
Targeted Allele Detail

Summary

• Symbol: Itga5^tm2Hyn
• Name: integrin alpha 5 (fibronectin receptor alpha); targeted mutation 2, Richard Hynes
• MGI ID: MGI:4818931
• Synonyms: alpha5^flox
• Gene: Itga5 Location: Chr15:103252713-103275190 bp, - strand Genetic Position: Chr15, 58.9 cM
• Alliance: Itga5^tm2Hyn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:161850
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An FRT-flanked neo cassette was inserted 5' to a loxP flanked exon 1 by homologous recombination. The selection cassette was removed by flp-mediated recombination. (J:159465, J:161850)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Itga5^tm2Hyn/Itga5^tm2Hyn	involves: 129S1/Sv * 129X1/SvJ
cn2	Itga5^tm2Hyn/Itga5^tm2Hyn Tg(Tek-cre)1Ywa/0	B6.Cg-Tg(Tek-cre)1Ywa Itga5^tm2Hyn
cn3	Itga5^tm1Hyn/Itga5^tm2Hyn Tg(H2-K1-tsA58)6Kio/0 Tg(Tek-cre)1Ywa/0	involves: 129 * C57BL/6 * C57BL/10 * CBA/Ca * SJL
cn4	Itga5^tm2Hyn/Itga5^tm2Hyn Itgav^tm2Hyn/Itgav^tm2Hyn Tg(H2-K1-tsA58)6Kio/0	involves: 129 * C57BL/6 * SJL
cn5	Itga5^tm1Hyn/Itga5^tm2Hyn Tg(Tek-cre)1Ywa/0	involves: 129 * C57BL/6 * SJL
cn6	Gt(ROSA)26Sor^tm1Sho/0 Itga5^tm2Hyn/Itga5^tm1Hyn Itgav^tm2Hyn/Itgav^+ Tg(Tek-cre)1Ywa/0	involves: 129 * C57BL/6 * SJL
cn7	Gt(ROSA)26Sor^tm1Sho/0 Itga5^tm2Hyn/Itga5^tm1Hyn Itgav^tm1Hyn/Itgav^tm2Hyn Tg(Tek-cre)1Ywa/0	involves: 129 * C57BL/6 * SJL

Phenotypes:

Affected Systems	cn1	cn2	cn3	cn4	cn5	cn6	cn7
show or hide all annotated terms
cardiovascular system		√				√	√
retroesophageal right subclavian artery							√
vascular ring							√
abnormal ascending aorta morphology							√
patent ductus arteriosus		√				√	√
ventricular septal defect						√	√
cellular	√	√	√	√		√	√
patent ductus arteriosus		√				√	√
abnormal cell adhesion			√	√
abnormal neuronal migration	√
mortality/aging		√				√	√
lethality throughout fetal growth and development, complete penetrance							√
postnatal lethality, incomplete penetrance		√				√
nervous system	√
abnormal neuronal migration	√
abnormal cerebral cortex morphology	√
normal phenotype					√
no abnormal phenotype detected					√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Itga5 Mutation: 47 strains or lines available

References

• Original: J:161850 van der Flier A, et al., Endothelial alpha5 and alphav integrins cooperate in remodeling of the vasculature during development. Development. 2010 Jul;137(14):2439-49
• All: 22 reference(s)