Npy^{tm1(rtTA,tetO-cre/EGFP)Snby}
Targeted Allele Detail

Summary

• Symbol: Npy^{tm1(rtTA,tetO-cre/EGFP)Snby}
• Name: neuropeptide Y; targeted mutation 1, Amanda Sainsbury
• MGI ID: MGI:4818970
• Synonyms: NPYCre
• Gene: Npy Location: Chr6:49799690-49806487 bp, + strand Genetic Position: Chr6, 24.04 cM, cytoband B3
• Alliance: Npy^{tm1(rtTA,tetO-cre/EGFP)Snby} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:162000
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase, Reporter, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutations: Insertion, Intragenic deletion
• Npy^{tm1(rtTA,tetO-cre/EGFP)Snby} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Npy (MGI:97374)

• Mutation details: The coding exons of the Npy gene were replaced with a cassette consisting of the reverse tetracycline transactivator (rtTA) gene, the EGFP gene in the opposite orientation, a neo selection cassette and cre recombinase controlled by the tetracycline responsive element (TRE; tetO), also in the opposite orientation to the Npy promoter. The stop codon of cre and the ATG initiation codon of EGFP were engineered to contain loxP sites in the same orientation so that cre mediated excision of the floxed region (including neo) would result in production of a cre/EGFP fusion product. Treatment with doxycycline activates the rtTA gene product, allowing transcription of the adjoining gene, which in turn excises the floxed segment. (J:162000)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Npy (mouse) Summary of all recombinase alleles driven by Npy. Alliance mouse Npy gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Npy Mutation: 19 strains or lines available

References

• Original: J:162000 Shi YC, et al., NPY neuron-specific Y2 receptors regulate adipose tissue and trabecular bone but not cortical bone homeostasis in mice. PLoS One. 2010;5(6):e11361
• All: 1 reference(s)