Pms2tm1.1Wed
Targeted Allele Detail
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Symbol: |
Pms2tm1.1Wed |
Name: |
PMS1 homolog2, mismatch repair system component; targeted mutation 1.1, Winfried Edelmann |
MGI ID: |
MGI:4819008 |
Synonyms: |
Pms2E702K, Pms2EK |
Gene: |
Pms2 Location: Chr5:143846782-143870786 bp, + strand Genetic Position: Chr5, 82.82 cM, cytoband G2
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Alliance: |
Pms2tm1.1Wed page
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Allele Type: |
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Targeted (Hypomorph) |
Mutations: |
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Insertion, Nucleotide substitutions
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Mutation details: An E702K mutation was introduced into exon 12 disrupting the DQHA(X)2E(X)4E metal binding motif. In addition, a floxed hygro/neo selection cassette was introduced in intron 12. Cre mediated recombination then removed the selection cassette. RT-PCR and qPCR indicted that expression levels of the mutant allele were similar to wild-type in MEFs and primary B cells. Western blot analysis indicated about a 25% reduction in protein levels in the spleens, brains, and testes of homozygous mice compared to wild-type tissues.
(J:162378)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Pms2 Mutation: |
53 strains or lines available
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Original: |
J:162378 van Oers JM, et al., PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13384-9 |
All: |
1 reference(s) |
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