Tg(NPHS2-icre/ERT2)1Dnsh
Transgene Detail

Summary

• Symbol: Tg(NPHS2-icre/ERT2)1Dnsh
• Name: transgene insertion 1, Farhad R Danesh
• MGI ID: MGI:4819592
• Synonyms: podocin-iCreER^T2
• Transgene: Tg(NPHS2-icre/ERT2)1Dnsh Location: unknown
• Alliance: Tg(NPHS2-icre/ERT2)1Dnsh page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(NPHS2-icre/ERT2)1Dnsh expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  NPHS2 (7827)

  Nphs2

  (MGI:2157018)

• Mutation details: The coding sequences of (improved) icre and a tamoxifen-inducible estrogen receptor, ER^T2, were fused in-frame and put under the control of 2.5 kb NPHS2 (human podocin) promoter region. The icre/ERT2 fragment was followed by the bovine growth hormone polyA (bGHpA) sequence. The linearized construct was microinjected into the pronuclei of fertilized C57BL/6 eggs. Three of four founders successfully transmitted the transgene to their offspring. (J:162633)
• Inheritance: Not Specified

Generation of Tg(NPHS2-icre/ERT2)1Dnsh.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	NPHS2 (human) Summary of all recombinase alleles driven by NPHS2. Comparative matrix view of recombinase activities Alliance human NPHS2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:162633 Wang J, et al., Tamoxifen-inducible podocyte-specific iCre recombinase transgenic mouse provides a simple approach for modulation of podocytes in vivo. Genesis. 2010 Jul;48(7):446-51
• All: 12 reference(s)