Tg(ATP6V1B1-cre)45Rnel
Transgene Detail

Summary

• Symbol: Tg(ATP6V1B1-cre)45Rnel
• Name: transgene insertion 45, Raoul Nelson
• MGI ID: MGI:4820423
• Synonyms: B1-Cre, VB1Cre
• Transgene: Tg(ATP6V1B1-cre)45Rnel Location: unknown
• Alliance: Tg(ATP6V1B1-cre)45Rnel page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(ATP6V1B1-cre)45Rnel expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  ATP6V1B1 (525)

  Atp6v1b1

  (MGI:103285)

• Mutation details: 7 kb of the 5' UTR of the human ATP6V1B1 gene (B1 promoter region) was cloned upstream of cre recombinase in the construct. The purified transgene fragment was injected into single-cell embryos. 50 founders were produced, and line 45 was analyzed further. Founders were bred onto a C57BL/6 x CBA background. Cre activity was assessed by crossing cre transgenic animals to mice carrying a floxed fluorescent reporter. Activity was observed in all intercalated cells in the collecting duct and in most cells of the connecting segment (including about half of the principal cells) and in the male reproductive tract, while low levels of activity were identified in non-ATP6V1B1 expressing tissues including the brain, large and small intestine, and uterus. No expression was detected in liver, stomach, skeletal muscle, heart or spleen. (J:162708)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	ATP6V1B1 (human) Summary of all recombinase alleles driven by ATP6V1B1. Comparative matrix view of recombinase activities Alliance human ATP6V1B1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:162708 Miller RL, et al., The V-ATPase B1-subunit promoter drives expression of Cre recombinase in intercalated cells of the kidney. Kidney Int. 2009 Feb;75(4):435-9
• All: 18 reference(s)