Tbx2^tm2.2Vmc
Targeted Allele Detail

Summary

• Symbol: Tbx2^tm2.2Vmc
• Name: T-box 2; targeted mutation 2.2, Vincent M Christoffels
• MGI ID: MGI:4822020
• Synonyms: Tbx2^delta2
• Gene: Tbx2 Location: Chr11:85723441-85732774 bp, + strand Genetic Position: Chr11, 51.34 cM
• Alliance: Tbx2^tm2.2Vmc page

Tbx2^tm2.2Vmc/Tbx2^tm2.2Vmc embryo shows duplication of digit IV on both hindlimbs

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163063
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A FRT flanked neo cassette with a 5' loxP site was inserted within intron 1 and an additional loxP site was inserted within intron 2 by homologous recombination. The selection cassette and exon 2 were removed by flpe and cre mediated recombination. Deletion of Exon 2 is expected to cause the loss of the domain essential for DNA binding and protein interactions and introduces a frame shift resulting in two stop codons in Exon 3 and early termination of translation. (J:163063)

Expression

In Mice Carrying this Mutation:	243 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbx2 Mutation: 29 strains or lines available

References

• Original: J:163063 Wakker V, et al., Generation of mice with a conditional null allele for Tbx2. Genesis. 2010 Mar;48(3):195-9
• All: 6 reference(s)