Ccn2^tm1.1Vlcg
Targeted Allele Detail

Summary

• Symbol: Ccn2^tm1.1Vlcg
• Name: cellular communication network factor 2; targeted mutation 1.1, Velocigene
• MGI ID: MGI:4822056
• Synonyms: Ctgf^e2COIN, Ctgf^tm1.1Anec
• Gene: Ccn2 Location: Chr10:24471340-24474581 bp, + strand Genetic Position: Chr10, 11.84 cM, cytoband A3-B1
• Alliance: Ccn2^tm1.1Vlcg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163027
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 was inserted in the antisense orientation into exon 2 via homologous recombination, splitting exon 2 into 2 new exons (120 and 103 bp in length). The two lox sites are in reverse complementation orientation, thus cre mediated recombination will irreversibly invert the inserted sequence. In addition, an FRT flanked hygromycin-delta-thymidine kinase cassette was inserted after the COIN intron. Flp mediated recombination removed the hygromycin cassette. Expression analysis confirmed expression of full length mRNA in homozygous mice. (J:163027)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccn2 Mutation: 29 strains or lines available

Notes

ES cells were F1H4 286A-B8 which carry the Ctgf^tm1Mga allele.

References

• Original: J:163027 Canalis E, et al., Connective tissue growth factor is required for skeletal development and postnatal skeletal homeostasis in male mice. Endocrinology. 2010 Aug;151(8):3490-501
• All: 4 reference(s)