Col1a1^{tm6(tetO-MSI2)Jae}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm6(tetO-MSI2)Jae}
• Name: collagen, type I, alpha 1; targeted mutation 6, Rudolf Jaenisch
• MGI ID: MGI:4822360
• Synonyms: Coll-TetO-MSI2
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm6(tetO-MSI2)Jae} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163322
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm6(tetO-MSI2)Jae} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MSI2 (124540)

  Msi2	(MGI:1923876)

• Mutation details: RMCE on KH2 cells inserted a cassette that contains a tetracycline operator and minimal CMV promoter drives inducible expression of the human cDNA preceded by a splice acceptor and polyadenylation sequence and followed by an FRT flanked PGK-neo cassette and promotorless hygro cassette into the 3'UTR. (J:163322)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:163322 Kharas MG, et al., Musashi-2 regulates normal hematopoiesis and promotes aggressive myeloid leukemia. Nat Med. 2010 Aug;16(8):903-8
• All: 4 reference(s)