S1pr1^tm1.1Thla
Targeted Allele Detail

Summary

• Symbol: S1pr1^tm1.1Thla
• Name: sphingosine-1-phosphate receptor 1; targeted mutation 1.1, Timothy Hla
• MGI ID: MGI:4822471
• Synonyms: S1p1r^S5A
• Gene: S1pr1 Location: Chr3:115504082-115508704 bp, - strand Genetic Position: Chr3, 50.0 cM
• Alliance: S1pr1^tm1.1Thla page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163389
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6)F1

Mutation description

Allele Type: Targeted Mutations: Insertion, Nucleotide substitutions Mutation details: Nucleotide substitutions in exon 2 result in the amino acid substitution of five serines with alanines (p.[Ser351Ala;Ser353Ala;Ser355Ala] and p.(Ser358_Ser359delinsAlaAla)). This prevents phosphorylization of the five residues and the produced protein fails to internalize correctly, but signaling is not disrupted. A loxP site flanked neomycin resistance gene cassette inserted into intron 2 was removed by subsequent cre-mediated recombination. (J:163389)

Generation of the S1pr1^tm1.1Thla allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any S1pr1 Mutation: 32 strains or lines available

References

• Original: J:163389 Thangada S, et al., Cell-surface residence of sphingosine 1-phosphate receptor 1 on lymphocytes determines lymphocyte egress kinetics. J Exp Med. 2010 Jul 5;207(7):1475-83
• All: 6 reference(s)