Adam17^tm1.1Srj
Targeted Allele Detail

Summary

• Symbol: Adam17^tm1.1Srj
• Name: a disintegrin and metallopeptidase domain 17; targeted mutation 1.1, Stefan Rose-John
• MGI ID: MGI:4822480
• Synonyms: ADAM17^ex
• Gene: Adam17 Location: Chr12:21373510-21423633 bp, - strand Genetic Position: Chr12, 8.3 cM
• Alliance: Adam17^tm1.1Srj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163380
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An FRT flanked neo with a 5' loxP site was inserted upstream of exon 11. A modified exon 11 (exon 11a) was inserted downstream of the endogenous exon. It contains a stop codon at the 5' end and a loxP site at the 3' end. Flp mediated recombination removed the neo cassette leaving exons 11 and 11a floxed. Severely reduced protein expression was confirmed by western blot analysis on skin and heart extracts. This is a hypomorphic allele. (J:163380)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Adam17^tm1.1Srj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adam17 Mutation: 64 strains or lines available

References

• Original: J:163380 Chalaris A, et al., Critical role of the disintegrin metalloprotease ADAM17 for intestinal inflammation and regeneration in mice. J Exp Med. 2010 Aug 2;207(8):1617-24
• All: 24 reference(s)