Jag2^tm2.2Grid
Targeted Allele Detail

Summary

• Symbol: Jag2^tm2.2Grid
• Name: jagged 2; targeted mutation 2.2, Tom Gridley
• MGI ID: MGI:4829505
• Synonyms: Jag2^del2
• Gene: Jag2 Location: Chr12:112871439-112893396 bp, - strand Genetic Position: Chr12, 61.37 cM
• Alliance: Jag2^tm2.2Grid page

Cleft palate, fused tongue, and syndactyly in Jag2^tm2.2Grid/Jag2^tm2.2Grid fetuses

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163652
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT flanked PGKneo cassette and a distal loxP site were inserted into intron 2 and a second loxP site was inserted about 1.1 kb upstream of the start site via homologous recombination. Cre mediated recombination removed exons 1 and 2. In situ hybridization analysis indicated absence of RNA expression in E10.5 homozygous embryos. RT-PCR analysis detected low levels of expression in homozygous mice. (J:163652)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Jag2 Mutation: 44 strains or lines available

References

• Original: J:163652 Xu J, et al., Generation of mice with a conditional null allele of the Jagged2 gene. Genesis. 2010 Jun;48(6):390-3
• All: 1 reference(s)