Rnf4^{Gt(ROSABetageo)1Gel}
Gene trapped Allele Detail

Summary

• Symbol: Rnf4^{Gt(ROSABetageo)1Gel}
• Name: ring finger protein 4; gene trap 1, Gary E Lyons
• MGI ID: MGI:4829855
• Gene: Rnf4 Location: Chr5:34493594-34510789 bp, + strand Genetic Position: Chr5, 17.87 cM
• Alliance: Rnf4^{Gt(ROSABetageo)1Gel} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163687
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The ROSA beta-geo gene trap vector inserted into the first intron of the gene. Northern blot analysis confirmed the absence of full length transcript expression in homozygous embryos. In situ hybridization analysis indicates that Xgal staining matches the endogenous gene expression pattern. (J:40477, J:163687)

Expression

In Mice Carrying this Mutation:	15 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rnf4 Mutation: 22 strains or lines available

References

• Original: J:163687 Hu XV, et al., Identification of RING finger protein 4 (RNF4) as a modulator of DNA demethylation through a functional genomics screen. Proc Natl Acad Sci U S A. 2010 Aug 24;107(34):15087-92
• All: 2 reference(s)