Nfkbia^tm1.1Pjc
Targeted Allele Detail

Summary

• Symbol: Nfkbia^tm1.1Pjc
• Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha; targeted mutation 1.1, Paul J Chiao
• MGI ID: MGI:4829878
• Synonyms: IkappaBalpha^M, IkBa^M
• Gene: Nfkbia Location: Chr12:55536195-55539432 bp, - strand Genetic Position: Chr12, 24.0 cM, cytoband C1-C3
• Alliance: Nfkbia^tm1.1Pjc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163686
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Two well defined NFKB binding sites (M1 and M2) and 4 NFKB like binding sites (M3 - M6) in the promoter region were mutated, a FLAG tag was attached to exon 1, and a floxed neo cassette was inserted in intron 1 via homologous recombination. Cre mediated recombination removed the neo cassette. The locations of and mutations in the 6 binding sites are as follows: -115 - -93, a TCC motif changed to a CCG (M1); -143 - -122, a CCC motif changed to an AGT (M2); -227 - -205, a TCT motif changed to a CAC (M3); -292 - -271, a CC motif changed to a AA (M4); -319 - -297, a GGG motif changed to a TTT (M5); -387 - -365, a CCC motif changed to a GGG (M6). Chromatin immunoprecipitation analysis confirmed the loss of kappa B enhancer function in MEFs from homozygous mice. (J:163686)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nfkbia Mutation: 19 strains or lines available

References

• Original: J:163686 Peng B, et al., Defective feedback regulation of NF-kappaB underlies Sjogren's syndrome in mice with mutated kappaB enhancers of the IkappaBalpha promoter. Proc Natl Acad Sci U S A. 2010 Aug 24;107(34):15193-8
• All: 3 reference(s)