Cyp1a1^tm2.1Dwn
Targeted Allele Detail

Summary

• Symbol: Cyp1a1^tm2.1Dwn
• Name: cytochrome P450, family 1, subfamily a, polypeptide 1; targeted mutation 2.1, Daniel W Nebert
• MGI ID: MGI:4830522
• Synonyms: Cyp1a1^mc
• Gene: Cyp1a1 Location: Chr9:57595211-57611107 bp, + strand Genetic Position: Chr9, 31.34 cM
• Alliance: Cyp1a1^tm2.1Dwn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163973
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 was replaced with a modified exon 2 in which nucleotide substitutions (AGA to GAC at codon 34 and AAA to ATA at codon 39) result in the amino acid substitutions of aspartic acid for arginine at position 34 (R34D) and isoleucine for lysine at position 39 (K39I) within the mitochondrial targeting domain. A floxed neo cassette inserted upstream of the modified exon was removed by cre mediated recombination. The product is predicted to undergo ER-specific targeting. (J:163973)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp1a1 Mutation: 39 strains or lines available

References

• Original: J:163973 Dong H, et al., Knock-in mouse lines expressing either mitochondrial or microsomal CYP1A1: differing responses to dietary benzo[a]pyrene as proof of principle. Mol Pharmacol. 2009 Mar;75(3):555-67
• All: 2 reference(s)