Kras^tm2(Kras)Rdl
Targeted Allele Detail

Summary

• Symbol: Kras^tm2(Kras)Rdl
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 2, Roberto Di Lauro
• MGI ID: MGI:4830590
• Synonyms: Kras4B KO, KrasKI
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm2(Kras)Rdl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98940
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A cassette containing Kras cDNA and a neo was fused in-frame with exon 2 such that synthesis of the endogenous Kras product would be abolished. The rearranged locus was designed to express only the main K-Ras 4B isoform and not the K-Ras 4A protein. (J:98940)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 84 strains or lines available

References

• Original: J:98940 Potenza N, et al., Replacement of K-Ras with H-Ras supports normal embryonic development despite inducing cardiovascular pathology in adult mice. EMBO Rep. 2005 May;6(5):432-7
• All: 3 reference(s)