Cend1^tm1.1Rmat
Targeted Allele Detail

Summary

• Symbol: Cend1^tm1.1Rmat
• Name: cell cycle exit and neuronal differentiation 1; targeted mutation 1.1, Rebecca Matsas
• MGI ID: MGI:4831137
• Gene: Cend1 Location: Chr7:141006359-141009404 bp, - strand Genetic Position: Chr7, 86.77 cM, cytoband F5
• Alliance: Cend1^tm1.1Rmat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164148
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region and 1 kb of the 3' UTR were replaced with a lacZ gene and a floxed PGK-neo cassette via homologous recombination. Cre mediated recombination removed the neo cassette. RT-PCR, in situ hybridization and western blot analysis confirmed the absence of RNA and protein expression in homozygous mice. Beta-galactosidase expression is detected in neurons in the cortex, dentate gyrus, striatum and cerebellum where the endogenous protein is highly expressed. Expression of beta-galactosidase is targeted to the nucleus. (J:164148)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cend1 Mutation: 5 strains or lines available

References

• Original: J:164148 Sergaki MC, et al., Impaired cerebellar development and deficits in motor coordination in mice lacking the neuronal protein BM88/Cend1. Mol Cell Neurosci. 2010 May;44(1):15-29
• All: 2 reference(s)