Gad1^tm2(cre)Tama
Targeted Allele Detail

Summary

• Symbol: Gad1^tm2(cre)Tama
• Name: glutamate decarboxylase 1; targeted mutation 2, Nobuaki Tamamaki
• MGI ID: MGI:4833682
• Synonyms: Gad1^tm1(cre)Tama, GAD67-Cre
• Gene: Gad1 Location: Chr2:70392109-70432358 bp, + strand Genetic Position: Chr2, 41.63 cM, cytoband D
• Alliance: Gad1^tm2(cre)Tama page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164349
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Gad1^tm2(cre)Tama expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gad1 (MGI:95632)

• Mutation details: A targeting vector was designed where the cre recombinase gene was inserted into immediately after the translational initiation site of the Gad1 (GAD67) gene in frame. This vector contained a 3-kb fragment at the 5' side, the cre gene inserted behind the Gad1 translational start, a Pgk-neo-p(A) cassette flanked by two Flp recognition target (FRT) sites, a 7-kb fragment at the 3' side, and an MC1 promoter-driven diphtheria toxin gene for selection. The vector was introduced into C57BL/6 ES cells and ES cells showing homologous recombination were injected into 8-cell stage CD-1 embryos. This allele is a null allele. (J:164349, J:173688)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gad1 (mouse) Summary of all recombinase alleles driven by Gad1. Comparative matrix view of recombinase activities Alliance mouse Gad1 gene page

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gad1 Mutation: 65 strains or lines available

References

• Original: J:164349 Higo S, et al., Subtypes of GABAergic neurons project axons in the neocortex. Front Neuroanat. 2009;3:25
• All: 12 reference(s)