Tg(Mcpt8-cre)1Voeh
Transgene Detail

Summary

• Symbol: Tg(Mcpt8-cre)1Voeh
• Name: transgene insertion 1, David Voehringer
• MGI ID: MGI:4834475
• Synonyms: Mcpt8Cre
• Transgene: Tg(Mcpt8-cre)1Voeh Location: unknown
• Alliance: Tg(Mcpt8-cre)1Voeh page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Mcpt8-cre)1Voeh expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mcpt8 (MGI:1261780)

• Mutation details: A vector containing a cre-loxP-neo-loxP cassette flanked by two short homologous sequences was electroporated into the bacteria (EL250) together with a modified BAC (RP23-16G10) containing the Mcpt8 locus. By homologous recombination, the coding sequence of cre recombinase was inserted directly behind the Mcpt8 start codon. The neo cassette was deleted by growing bacteria in arabinose-containing medium and the BAC clone was injected into the pro-nucleus of fertilized C57BL/6 oocytes. The copy number of the integrated BAC in two different founder lines was determined by quantitative PCR for Mcpt8 (5 copies in line #1 and 7 copies in line #2.) (J:164432)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mcpt8 (mouse) Summary of all recombinase alleles driven by Mcpt8. Alliance mouse Mcpt8 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:164432 Ohnmacht C, et al., Basophils orchestrate chronic allergic dermatitis and protective immunity against helminths. Immunity. 2010 Sep 24;33(3):364-74
• All: 6 reference(s)