Clcn5^tm5.1Tjj
Targeted Allele Detail

Summary

• Symbol: Clcn5^tm5.1Tjj
• Name: chloride channel, voltage-sensitive 5; targeted mutation 5.1, Thomas J Jentsch
• MGI ID: MGI:4834485
• Synonyms: Clcn5^Y672E*
• Gene: Clcn5 Location: ChrX:7020049-7185597 bp, - strand Genetic Position: ChrX, 3.21 cM, cytoband F3-F4
• Alliance: Clcn5^tm5.1Tjj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164474
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Not Specified) Mutations: Insertion, Nucleotide substitutions Mutation details: A loxP site was inserted downstream of exon 9, exon 11 was replaced with one containing nucleotide substitutions that result in the amino acid substitution of glutamic acid for tyrosine at position 672 (Y672E), and exon 12 was replaced with a modified exon 12 in which nucleotide substitutions result in the amino acid substitutions alanine for aspartic acid and methinone for leucine. The mutations in exon 12 abolish antibody recognition of the C-terminus by converting it to resemble the C-terminus of Clcn3. This provided a tag to differentiate the modified protein from wild-type. An FRT flanked neo cassette inserted downstream of the modified exon 11 was removed by flp mediated recombination. (J:164474)

Generation of the Clcn5^tm5.1Tjj allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clcn5 Mutation: 17 strains or lines available

References

• Original: J:164474 Rickheit G, et al., Role of ClC-5 in renal endocytosis is unique among ClC exchangers and does not require PY-motif-dependent ubiquitylation. J Biol Chem. 2010 Jun 4;285(23):17595-603
• All: 1 reference(s)